Hao Zhou


Bachelor, Guizhou medical university (貴州医科大学), China

Master, Zunyi medical university (遵義医科大学), China


1. TCR and BCR repertoire in Chiroptera

During my master's course, I undertook a project involving genome annotation and the analysis of new species' TCR repertoires. Bats, being unique in their ability to coexist with a wide array of viruses and pathogens, present a fascinating case for studying adaptive immune responses to invasive antigens, about which little is currently known. My initial task involved annotating bat TCR loci from genomic data(ZHOU, 2021). This allowed us to establish a reference library for analyzing raw data using MiXCR. Subsequently, I collected spleen samples from bats for TCR sequencing. This led to a comparative study of the TCR repertoire's basic features between bats and model species such as humans and mice, marking the first exploration into the immunological characteristics of Chiroptera(ZHOU, 2023).

bat CDR3 repertoire.jpeg

2. Annotation of adaptive immune receptor gene in eutherian genomes

After joining Daron's lab, I began collaborating with Professor Kazutaka Katoh, inspired by my prior research experiences. Recognizing the limitations of manual annotation—its time-consuming nature and the challenge of identifying all potential genes—my current project focuses on developing a tool or methodology for annotating adaptive immune genes across the genomes of different species. This project combines conventional methods, such as local alignment, with advanced machine learning algorithms to achieve more precise predictions.Poster.jpeg


  1. Hao ZHOU1#, et al (2021). TR Locus Annotation and Characteristics of Rhinolophus ferrumequinum. Front. Immunol. 12:741408. doi: 10.3389/fimmu.2021.741408
  2. Hao ZHOU, Long Ma, et al (2021). Overview and progress of studies on virus carrying mechanism in bats. Chinese Journal of Immunology. 37(20):2539-2543. Doi: 10.3969/j.issn.1000-484X.2021.20.022
  3. Xu Z, Ismanto HS, Hao ZHOU, Saputri DS, Sugihara F and Standley DM (2022), Advances in antibody discovery from human BCR repertoires. Front. Bioinform. 2:1044975. doi: 10.3389/fbinf.2022.1044975
  4. Hao ZHOU, et al (2023). New insights into the Germline Genes and CDR3 Repertoire of the TCRβ chain in Chiroptera. Front. Immunol. 14:1147859. doi: 10.3389/fimmu.2023.1147859
  5. Long Ma, Longyu Liu, Jun Li, Hao ZHOU, et al (2024) Landscape of IGH germline genes of Chiroptera and the pattern of Rhinolophus affinis bat IGH CDR3 repertoire. Microbiology Spectrum doi: https://doi.org/10.1128/spectrum.03762-23